Samira A Abdullahi, Helen Ehimemen, Nkechi Nyim


Medicinal plants are the most affordable source of disease treatment in Africa. Different parts of plants are screened for their curative and disease management of various illnesses. Cassia sieberiana (D.C) is a medicinal plant widely distributed in the Southern Sahel, Sudan Savanna and distributed in the semi-arid north eastern part of Nigeria. It is a source of some bioactive compounds and has an antioxidant activity. This study was aimed to determine in-vitro antioxidant and hemin biomineralization inhibitory properties of Cassia sieberiana (D.C). Aqueous stem bark extract. Phytochemicals screening and in vitro free radical scavenging activity of aqueous stem bark extract of Cassia sieberiana D. C was conducted using 3 – 5 weeks old albino rats .Results showed the presence of carbohydrates, alkaloids, tannins, flavonoids, saponins and steroids. The extract displayed significant scavenging activity of DPPH at higher concentration doses (9mg/ml). The extract displayed non-hemolytic activity at high concentrations, with the maximum inhibition at 8mg/ml, while the inhibition of hemolysis induced by PHZ (phenylhydrazine) was dose dependent, with a significant increase in erythrocyte membrane protection at 10mg/ml, with IC50 at 1.729 mg/ml. The extract displayed potential antimalarial activity against the bio-mineralization of heme, increased inhibition was exhibited with increased concentration (IC50 =5.808). These findings suggest that the aqueous extract of Cassia sieberiana possesses antimalarial properties likely mediated due to the presence of secondary metabolites and its ability to scavenge free radicals prevents the bio-mineralization of heme. Thus, it can be further explored for the effective management of malaria.

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Abdulrazak, N., Asiya, U. I.,Usman, N. S., Unata, I. M., and Farida, A (2015). Anti-plasmodial activity of ethanolic extract of root and stemback of Cassia sieberiana DC on mice. Journal of Intercultural Ethnopharmacology, 4(2):96–101.

Ali, K.,Fulya, T.,Artun,G., Özcan,G., Melikoğlu,S., Anıl,Ş., Kültür and Nurhayat S (2015). In vitro evaluation of antioxidant activity of some plant methanol extracts. Jounal of biotechnology and biotechnological equipment, 29(6):1184-1189

Altemimi, A., Lakhssassi, N., Baharlouei, A., Watson, D. G and Lightfoot, D. A (2017). Phytochemicals: Extraction, Isolation, and Identification of Bioactive Compounds from Plant Extracts. Plants (Basel, Switzerland), 6(4): 42.

Atanasov, A. G., Waltenberger, B., Pferschy-Wenzig, E. M., Linder, T., Wawrosch, C., Uhrin, P and Stuppner, H (2015). Discovery and resupply of pharmacologically active plant-derived natural products: A review. Biotechnology advances, 33(8): 1582–1614.

Autino, B., Noris, A., Russo, R and Castelli, F(2012). Epidemiology of malaria in endemic areas. Mediterranean journal of hematology and infectious diseases, 4(1).

Claro, l., Maria,s.s.l.,Samuel,R.C. and guinaldo J.N (2006). Effect of vitamins C and E on oxidative processes in human erythrocytesCell Biochemistry and Function, 24(6):531

David, A.F. Philip, J.R. Simon, L.C. Reto, B and Solomon, N (2004). Nature Reviews, 3: 509-520.

Donkor, K., Stephen, A., Jerry, A., Nutifafa, T., Nii, O.M. and Laud, K.O (2013). Analgesic and anti-inflammatory activities of Asena, a herbal preparation for treatment of arthritis, using rodent models. Medicinal and Aromatic Plant Research Journal, 1(2): 20-29

Guha M., Kumar S., Choubey V., Maity P and Bandyopadhyay, U (2006). Apoptosis in liver during malaria: Role of oxidative stress and implication of mitochondrial pathway. FASEB Journal, 20:439–449.

Gurib-Fakim, G (2006). Medicinal plants: traditions of yesterday and drugs of tomorrow. Molecular Aspects of Medicine, 27: 1-93.

Karimi, A., Majlesi, M and Rafieian-Kopaei, M (2015). Herbal versus synthetic drugs; beliefs and facts. Journal of nephropharmacology, 4(1): 27–30.

Kasote, D. M., Katyare, S. S., Hegde, M. V and Bae, H (2015). Significance of antioxidant potential of plants and its relevance to therapeutic applications. International journal of biological sciences, 11(8): 982–991.

Kumar S, Guha M, Choubey V, Maity P and Bandyopadhyay, U (2007). Antimalarial drugs inhibiting hemozoin (beta-hematin) formation: a mechanistic update. Life Sci, 80(9):813-28.

Mary G., Ewura S.Y and Vanessa, S (2018). African Herbal Remedies with Antioxidant Activity: A Potential Resource Base for Wound Treatment,” Evidence-Based Complementary and Alternative Medicine, p 58

Obidah W., Saad, U.A and Wurochekke, A.U(2009). Toxic effects of aqueous stem bark extract of Cassia sieberiana on some biochemical parameters in rats. African Journal of Biochemistry Research, 3(5):229-231

Okokon, J.E. Ita, B.N and Udokpoh, A.E (2006). Annals of Tropical Medicine and Parasitology 100, 585–590.

Odikamnoro, O. O., Ikeh, I. M., Okoh, F. N., Ebiriekwe, S. C., Nnadozie, I. A., Nkwuda, J. O., and Asobie, G. C (2017). Incidence Of Malaria/Typhoid Co-Infection Among Adult Population In Unwana Community, Afikpo North Local Government Area, Ebonyi State, Southeastern NIGERIA. African journal of infectious diseases, 12(1): 33–38.

Pizzino, G., Irrera, N., Cucinotta, M., Pallio, G., Mannino, F., Arcoraci, V and Bitto, A (2017). Oxidative Stress: Harms and Benefits for Human Health. Oxidative medicine and cellular longevity ,8416763.

Sadeghi,Z., Valizadeh,J.,Azyzian,O and Akaberi, M (2015). Antioxidant activity and total phenolic content of Boerhavia elegans (choisy) grown in Baluchestan, Iran.Avicenna J Phytomed. Vol 5(1): 1–9.

Sahoo, S., Ghosh, G., Das, D and Nayak, S (2013). Phytochemical investigation and in vitro antioxidant activity of an indigenous medicinal plant Alpinia nigra B.L. Burtt. Asian Pacific Journal of Tropical Biomedicine, 3(11): 871–876.

Sharareh, R, Hamid, P and Javad, J (2015). Antioxidant properties of several medicinal plants growing wild in northeastern Iran. Asian Journal of Plant Science and Research, 5(2):63-6

Shittu, A.O., Oyi,A.R., and Onaolapo, J.A (2010). Isolation, Characterisation and Compaction Properties of Acacia sieberiana Gum in Chloroquine and Metronidazole Tablet Formulation. International Journal of Pharmaceutical and Biomedical Research, 1(4); 149-153.

Sofowora A (1993). Medicinal plants and Traditional Medicine in Africa. Spectrum books, Ibadan 1, pp.150.

Trease GE, Evans WC (1989). Pharmacognosy 13th ed. Bailliere Tindall, London. pp


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