GC-MS ANALYSIS AND ANTIBACTERIAL PROPERTIES OF GUM EXUDATES OF Anogeissusleiocarpus D.C

Sani Haruna, I. A. Sani, B. S. Aliyu, G. Ibrahim, M. S. Mohammed

Abstract


This  investigation  was  carried  out  to  evaluate  the  phytochemical  constituents by  GC-MS   analysis  and  determine  the  antibacterial  properties  of  gum  exudate of  Anogeissusleiocarpus.The GC-MS  analysis  of  the  ethanolicgum extracts  of  A. leiocarpuswas  performed  using  a  Perkin-Elmer GC clarus  500  system.The antibacterial  properties  of  the  gum  exudatewas  determine  by  disc  diffusion  method. The GC-MS  analysis  revealed  the  presence  of  various  phytochemicalsin  the  gum extracts  with  the  varying  degree  of  biological  activities.  From  the  result,  Fourteen (14)  compounds  were  identified  with  1H-3a-7, methanoazulene  (14.31%), Alloaromadendrene(12.24%)  being  the  most abundant  compounds  identified.  Other  compounds  were,  1-4( 4-pyrimidine-2-yl) piperazin-1-yl peropane-1-one,  2,5- dimethyl-3-methylene,1,5-hexadiene, 11,-(2-cyclopentene-1-yl undecanoic acid, 1,6,10,-dodecatriene,-7,11-dimethyl-3-methylene,  Bicyclo[5.1.0] octane, 8-(1-methylthyline)-,  Docosa-8,14-dyn-1,2,2-diol (Z)- 2Tms derivatives, Polygalitol,  Morpholine,4-methyl-4,oxide,  Carbamodithioic acid, formylMethyl ester.Results  of  antibacterial  properties  showed  that,   the  gum  extracts  have  shown  moderate  activity  on  the  test  organisms.  From  the   results,  chloroform  and  ethanolic  extract have significant    activity  against  all  the  test  organisms.  On  the  effect  of  concentrations  of  the  extracts,  the  control  disc  significantly  recorded    the  highest  activity  in  all  the  test  organisms  (P≤0.05)   followed  by  5000µg/disc  and  3000 µg/disc  of  the  crude  extract.  The  activity  exhibited  by  the  extracts  of  the  gum  sample  justified  the  use  of  gum  exudate  as  a  good  source  of  modern  and  alternative  medicine. Result of GCMS analysis will  greatly  be  helpful  for  the  discovery  of  potent  remedies  for  various  diseases.

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