PREVALENCE OF LISTERIA SPECIES IN BLUE WHITING (MICROMESISTUS POUTASOU) IN LAGOS STATE, NIGERIA.

Esther Esak Amusan, Sanni ABIODUN I, Banwo Kolawole Kolawole

Abstract


Listeria species are ubiquitous in nature. The ubiquity of Listeriae enables them to colonize and persist in the food-processing environments and the food chain. Blue Whiting also known as panla is a pelagic fish that is widely consumed in Lagos and its environs.  The present study examined the prevalence of Listeria spp. from Blue Whiting sold at three retail markets in Lagos State. One hundred and twenty-four Blue Whiting samples consisting of fifty-three raw fish and seventy-one smoked fish were microbiologically analyzed using the Oxoid Listeria Précis method, Oxoid Listeria Test Kit and MICROBACT Listeria 12L system. Genotypic identification was carried out using 16S rRNA gene sequencing. Of the 124 samples examined, 21(16.9%) were positive for Listeria spp. with 18 (14.5%) identified as L. innocua and 3 (2.4%) as L. grayi. Higher occurrences (14-24%) of Listeria spp. were observed between July and November, 2016. Fourteen out of 18 isolates selected showed amplification for the 16S rRNA region of Listeria spp. Sanitary conditions and personal hygiene should be adhered to during processing, distribution and marketing of Blue Whiting so as to reduce contamination by Listeria species since the presence of L. innocua serves as an indicator for L. monocytogenes


Full Text:

PDF

References


Adebayo-Tayo, A.C., Odu, N.N., Michael, M.U. and Okonko, I.O. (2012). Multi-Drug Resistant (MDR) Organisms isolated from Sea-foods in Uyo, South-Southern Nigeria. Nature and Science, 10(3): 61-70.

Adeshina, I. and Adewale, Y.A. (2015). Preliminary studies on the prevalence of Listeria monocytogenes in Clarias gariepinus and Orechromis niloticus in Ibadan, Oyo State. J. Agric. Res. Dev. 14(1): 45-51.

Adeshina, I., Abdulwahab, M., Adewale, Y.A., Suleiman, S.B. and Tiamiyu, L.O. (2017). Detection of Listeria monocytogenes in Fried Fish, Processing Slab and Tools in Kwara State, Nigeria. Harran Üniv. Vet. Fak. Derg. 6(1): 32-37.

Adeyeye, S.A.O., Oyewole, O.B., Obadina, A.O., Omemu, A.M., Adeniran, O.E., Oyedele, H.A. and Abayomi, S.O. (2015). Quality and Safety Assessment of Traditional Smoked Fish from Lagos State, Nigeria. International Journal of Aquaculture, 5(15):1-9.

Ajayeoba, T.A., Atanda, O.O., Obadina, A.O., Bankole, M.O. and Adelowo, O.O. (2016). The incidence and distribution of Listeria monocytogenes in ready-to-eat vegetables in South-Western Nigeria. Food Science and Nutrition, 4(1): 59-66.

Amusan, E.E. (2012). Detection of Listeria monocytogenes in frozen fish in Lagos, Nigeria. In the Proceedings of the 27th Annual Conference and Biennial General Meeting of the Fisheries Society of Nigeria held at Banquet Hall Government House/Faculty of Law, Niger-Delta University, Bayelsa State, 25th-30th November, pp: 307-310.

Amusan, E.E., Sanni, A.I. and Banwo, K. (2017). Listeria Species in Seafoods from two Major Fish Markets in Lagos, Nigeria. Covenant Journal of Physical and Life Sciences, 5(1):52-58.

Camargo, A.C., Woodward, J.J. and New, L.A. (2016). The continuous challenge of characterizing the foodborne pathogen Listeria monocyogenes. Foodborne Pathogens and. Disease 13(8):405-416.

Chakravorty, S., Helb, D., Burday, M., Connell, N. and Alland, D. (2007). A detailed analysis of 16S ribosomal RNA gene segments for the diagnosis of pathogenic bacteria. Journal of Microbiological Methods, 69(2):330–339.

Chukwu, O.O.C., Ehizibolo, O.D., Mustapha, A.I., Chukwu, D.I., Olabode, O.A. and Ogbonna, C.I.C. (2006). Listeria species in Smoked Fish on the Retail Markets of Jos, Nigeria. Animal Production Research Advances, 2(4): 231-234.

Dalzini, E., Bernini, V., Bertasi, B., Daminelli, P., Losio, M.N. and Varisco, G. (2015). Survey of prevalence and seasonal variability of Listeria monocytogenes in raw cow milk from Northern Italy. Food Control. http://dx.doi.org/10.1016/ j.foodcont.2015.08.019.

Das, S., Lalitha, K.V., Thampuran, N. and Surendran, P.K. (2013). Isolation and characterization of Listeria monocytogenes from tropical seafood of Kerala, India. Annals of Microbiology, 63:1093 –1098.

Eruteya, O.C., Odunfa, S.A. and Lahor, J. (2014). Listeria spp. in raw cow and goat meat in Port Harcourt, Nigeria. British Biotechnology Journal, 4(2): 204-214.

Gasanov, U., Hughes, D. and Hansbro, M.P. (2005). Methods for the isolation and identification of Listeria spp. and Listeria monocytogenes: a review. FEMS Microbiology Reviews, 29: 851- 875.

Gómez, D., Ariño, A., Carramiñana, J.J., Rota, C. and Yangüela, J. (2012). Sponge versus mini-roller for the surface microbiological control of Listeria monocytogenes, total aerobic mesophiles and Enterobacteriaceae in the meat industry. Food Control. 27: 242-247.

Hadjilouka, A., Andritsos, N.D., Paramithiotis, S., Mataragas, M. and Drosinos, E.H. (2014). Listeria monocytogenes serotype prevalence and biodiversity in diverse food products. Journal of Food Protection, 77(12): 2115-2120.

Hellberg, R.S., Martin, K.G., Keys, A.L., Haney, C.J., Shen, Y.and Smiley, R.D. (2013). 16S rRNA partial gene sequencing for the differentiation and molecular subtyping of Listeria species. Food Microbiology, 36(2): 231–240

Hitchins, A.D. and Jinneman K. (2011). Detection and Enumeration of Listeria monocytogenes in Foods. Chapter 10. In: Bacteriological Analytical Manual. U.S. Food and Drug Administration, Silver Spring.

Ikeh, M.A.C., Obi, S.K.C., Ezeasor, D.N., Ezeonu, I.M. and Moneke, A.N. (2010). Incidence and pathogenicity profile of Listeria sp. isolated from food and environmental samples in Nsukka, Nigeria. African Journal of Biotechnology, 9(30): 4776-4782.

Jami, M., Ghanbari, M., Zunabovic, M., Domig, K.J. and Kneifel, W. (2014). Listeria monocytogenes in Aquatic Food Products - A Review. Comprehensive Reviews in Food Science and Food Safety, 13: 798–813.

Jarvis, N.A., O’Bryan, C.A., Ricke, S.C., Johnson, M.G. and Crandall, P.G. (2016). A review of minimal and defined media for growth of Listeria monocyogenes. Food Control, 66: 256-269.

Karthikeyan, R., Gunasekaran, P. and Rajendhran, J. (2015). Molecular serotyping and pathogenic potential of Listeria monocytogenes isolated from milk and milk products in Tamil Nadu, India. Foodborne Pathogens and Disease. 12(6): 522-528. http://dx.doi.org/10.1089/fpd.2014.1872.

Kaur, S., Singh, R., Sran, M.K. and Gill, J.P.S. (2017). Molecular Characterization of Listeria monocytogenes in white meat samples form Punjab, India. Indian Journal of Animal Research, 52(11): 1635-1641.

Kolade, O.Y. (2015). Fatty Acid Profile Investigation of Blue Whiting Fish (Micromesistius poutasou) flesh from Agbalata Market Badagry, Lagos West, Nigeria. Emerging Life Science Research, 1(2): 20-25.

Mohamed, Y., Reda, W.W., Abdel-Moein, K., Abd El-Razik, K.A., Barakat, A.M.A., El Fadaly, H.A., Hassanain, N.A. and Hegazi, A.G. (2016). Prevalence and phylogenetic characterization of Listeria monocytogenes isolated from processed meat marketed in Egypt. Journal of Genetic Engineering and Biotechnology, 14: 119–123.

Montero, D., Bodero, M., Riveros, G., Lapierre, L., Gaggero, A., Vidal, R.M. and Vidal, M. (2015). Molecular epidemiology and genetic diversity of Listeria monocytogenes isolates from a wide variety of ready-to-eat foods and their relationship to clinical strains from listeriosis outbreaks in Chile.

Frontiers in Microbiology, 6:384. doi:10.3389/fmicb.2015.00384.

Nayak, D.N., Savalia, C.V., Kalyani, I.H., Kumar, R. and Kshirsagar, D.P. (2015). Isolation, identification and characterization of Listeria spp. from various animal origin foods. Veterinary. World, 8(6): 695-701.

Nwachukwu, V.N. and Madubuko, C.U. (2013). Microflora associated with processing and storage of the white catfish (Chrysichthys nigrodigitatus). Journal of Fisheries and Aquatic Sciences, 8: 108-114.

Osimani, A. and Clementi, F. (2016). The occurrence of Listeria monocytogenes in mass catering: An Overview in the European Union. International Journal of Hospital Management, 57:9-17.

Park, S., Jung, J., Choi, S., Oh, Y., Lee, J., Chae, H., Ryu, S., Jung, H., Park, G., Choi, S., Kim, B., Kim, J., Chae, Y.Z., Jung, B., Lee, M. and Kim, H. (2012). Molecular Characterization of Listeria monocytogenes based on the PFGE and RAPD in Korea. Advances in Microbiology, 2: 605-616.

Rezai, R., Elham, A. and Behnam, S. (2018). Prevalence and Antimicrobial Resistance Profile of Listeria Species isolated from Farmed and On-Sale Rainbow Trout (Oncorhynchus mykiss) in Western Iran. Journal of Food Protection, 81(6): 886-891.

Salihu, M.D., Junaidu, A.U., Manga, S.B., Gulumbe, M.L., Magaji, A.A., Ahmed, A., Adamu, A.Y., Shittu, A. and Balarabe, I. (2008). Occurrence of Listeria monocytogenes in smoked fish in Sokoto, Nigeria. African Journal Biotechnology, 7: 3082-3084.

Shinkafi, S.A. and Ukwaja, V.C. (2010). Bacteria associated with fresh tilapia fish (Oreochromis niloticus) sold at Sokoto central market in Sokoto, Nigeria. Nigerian Journal of Basic and Applied Sciences, 18: 217-221.

Todd, E.C.D. and Notermans, S. (2011). Surveillance of listeriosis and its causative pathogen, Listeria monocytogenes. Food Control. 22: 1484-1490.

Vinothkumar, R., Arunagiri, K. and Sivakumar, T. (2013). Studies on pathogenic Listeria monocytogenes from marine food resources. Int. J. Curr. Microbiol. App. Sci. 1(1): 86-93.

Wang, X., Lu, X., Yin, L. and Liu, S. (2013). Occurrence and antimicrobial susceptibility of Listeria monocytogenes isolates from retail raw foods. Food Control. 32(1): 153-158.

Woo, P.C., Lau, S.K., Teng, J.L., Tse, H. and Yuen, K.Y. (2008). Then and now: use of 16S rDNA gene sequencing for bacterial identification and discovery of novel bacteria in clinical microbiology laboratories. Clin. Microbiol. Infect. 14(10):908–934.


Refbacks

  • There are currently no refbacks.




FEDERAL UNIVERSITY DUTSIN-MA, KATSINA STATE - Copyright 2019