EFFECT OF UPPERCOTT EXPOSURE ON THE LIVER OF FEMALE ALBINO WISTAR RATS

Emmanuel Uchechukwu Modo, B. O. Orji, I. Wopara, M. A. Agiang, F. E. Uboh

Abstract


Improving technology has led to more dependence on agricultural pesticides and herbicides in the fight against pests. These pesticides have been shown to have harmful effects on man and other species. In this study, the hepatic effect of exposure to cypermethrin and dimethoate mixture (uppercott) was investigated in female albino wistar rats. Thirty (30) female wistar albino rats were distributed into five (5) groups of six (6) animals each. Groups 1 and 2 served as the normal control and oil control respectively, groups 3, 4 and 5 received 2.5%, 5% and 7.5% LD50 of uppercott orally for 28 days. Results obtained revealed that uppercott exposure significantly (p<0.05) increased serum aspartate aminotransferase (AST) activity, alanine aminotransferase (ALT) activity and alkaline phosphatase (ALP) activity when the test groups were compared with control. Also, uppercott exposure raised serum bilirubin concentration slightly insignificantly (p>0.05) compared to the control. Superoxide dismutase (SOD) activity was significantly (p<0.05) reduced across all test groups as compared with compared with control. Histology of liver tissues revealed patchy necrotic sessions in the liver tissues of the test experimental groups (2.5%, 5% and 7.5%). The results obtained from this study are strongly indicative of the hepatotoxic effect of uppercott pesticide and hence, caution during usage is advised.

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Ahmed, M. A. and Mohamed, S. A. (2010). Dimethoate-induced hepatotoxicity in rats and the protective roles of Vitamin E and N-acetylcysteine. Egypt. J. Exp. Biol. (Zool.), 6(2): 219 – 230

Carvalho, F. P. (2017). Pesticides, environment, and food safety. Food Energy Secur., 6(2): 48-60.

Dimitrova, M. S., Tsinova, V. and Velcheva, V. (1994). Combined effect of Zinc and Lead on the hepatic superoxide dismutase–catalase system in carp (Cyprinus carpio). Comp. Biochem. Physiol., 108(1): 43–46.

Escobar, J. A., Rubio, M. A. and Lissi, E. A. (1996). SOD and catalase inactivation by singlet oxygen and peroxyl radicals. Free Radical Biol. Med., 20(3): 285–290.

Gomaa, M. S., AbdAlla, M. A and Sameer, M. M. (2011). The possible protective effect of propolis (Bee glue) on cypermethrin-induced hepatotoxicity in adult albino rats. Mansoura J. Forensic Med. Clin. Toxicol. XIX(1): 17–32.

IPCS and INCHEM. 2010. Chemical safety information from intergovernmental organizations, Geneva, World Health Organization, International Programme on Chemical Safety.

Jendrassik, L., and Grof, P. (1938). Colorimetric method of determination of bilirubin. Biochem. Z. 297:81-82.

Li, S., Tan, H., Wang, N., Zhang, Z., Lao, L., Wong, C. and Feng, Y. (2015). The role of oxidative stress and antioxidants in liver diseases. Int. J. Mol. Sci. 16 (11), 26087–26124.

Muthuviveganandavel, V., Muthuraman, P., Muthu, S. and Srikumar K. (2008). A study on low dose cypermethrin induced histopathology, lipid peroxidation and marker enzyme changes in male rat. Pest Bichem Physiol. 91:12–6.

Navarro, V. J. and Senior, J. R. (2006). Drug-related hepatotoxicity. N Engl J Med. 354:731-739.

Occupational Health Services, Inc. (1991). MSDS for dimethoate. OHS Inc. Secaucus, NJ.

Paoletti, F., Aldinucci, D., Mocali, A., and Caparrini, A. (1986). A sensitive spectrophotometric method for the determination of superoxide dismutase activity in tissue extracts. Analytical Biochemistry; 154: 538–541. https://doi.org/10.1016/0003-2697(86)90026-6.

Sami, I. A., Alaa, A. G., Amr, A. A., Sherien, M. E., Mona, E. and Jihan, H. (2018). Mitigation of Alpha-Cypermethrin-Induced Hepatotoxicity in Rats by Tribulus terrestris Rich in Antioxidant Compounds. Jordan Journal of Biological Sciences. 11(5): 517 – 525

Schlebusch, H., Rick, W., Lang, H., and Knedal, M. (1974). Standards in the activities of clinically important enzymes. Dtsch Med Inochenschr; 99: 765 - 766. https://doi.org/10.1055/s-0028-1107840.

Seven, A., Guzel, S., Seymen, O., Civelek, S., Bolayirh, M., Uncu, M. and Bur, G. (2004). Effects of vitamin E supplementation on oxidative stress in streptozotocin induced diabetic rats: investigation of liver and plasma. Yonsei Med. J. 45: 703–710.

Srivastava, A., Srivastava, M. K. and Raizada, R. B. (2006) Ninety day toxicity and one generation reproduction study in rats exposed to allethrin based LMR. J Toxicol Sci. 31:1–7.

Srivastava, B. D., Srivastava, M., Srivastav, S. k., Suzuki, N. and Srivastav, A. K. (2018). Cypermethrin-Induced Liver Histopathology in Rat: Protective Role of Jamun Seed and Orange Peel Extracts. Iranian Journal of Toxicology 12(4):25-30.

Tao, T. Y., Wei, L. Z., Yang, Y., Tao, Z. and Zhwo, Y. (2008) Effects of alpha and theta cypermethrin insecticide on transient outward potassium current in rat hippocampal CA3 neurons. Pesticide Biochem Physiol. 2008;90:1–7.

Thefeld, W., Hoffmeister, H., Busch, E. W., Koller, P, U., and Vollmar, J. (1974). Reference values for the determination of GOT, GPT and alkaliphosphatase in serum with optimal standard methods. Dtsch. Med. W ochenschr; 99:343 - 351. https://doi.org/10.1055/s-0028-1107760.

U. S. Environmental Protection Agency (EPA) (1998). Extremely hazardous substances (EHS) Chemical Profiles and Emegency First Aid Guides. Washington D.C.: U. S. Government Printing Office.


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