Ejiro Akponah


Toxicity of three new and locally synthesized dyes 3,5- dimethoxypyrimidine azo-6-methyl uracil (Dye 1);  4-ethyl 5,2,3-thiazole azo-6-methyl uracil (dye 2) and 5-ethoxybenzothiazole azobenzoloxyphenol (dye 3) to five soil borne fungi: Aspergillus niger, Fusarium sp, Trichoderma viride, Alternaria alternate and Penicillium chrysogenum was investigated. Result showed mycelia extension decreased with increase in dyes concentrations (at 0.1, 1.0, 10 and 100 mg/L). At 0.1mg/L, mycelia extensions were 11.5, 13.2, 22.9, 15.8 and 15.5 (mm) for T.  viride, A. alternate, P. chrysogenum A. niger and Fusarium sp. respectively. Lower mycelia lengths of 5.5mm (T.  viride), 3.17mm (A. alternate),2.55 mm ( P. chrysogenum), 14.5mm (A. niger) and 12.5mm ( Fusarium sp) were observed at highest concentration of 100mg/L . While response of five fungi to dyes did not differ, mycelia extension decreased with concentrations for all dyes (p ≤ 0.05). Additionally, A. niger and Fusarium sp  displayed capabilities of decolourizing  representative dye (dye 3) selected for decolourization test. At the end of a four day exposure period, percentage decolourization of dye at concentrations of 0.1,1.0, 10 and 100 mg/L were 60, 60.97, 19.96 and 10.26 (%) respectively. Decolourization abilities and the adaptation of mycelia growth to toxic pressure of dyes, suggest possible roles soil fungi could play in ameliorating effects of these dyes upon discharge into the environment.

Key words: Fungi, Dye, Toxicity, Decolourization

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Almieda, E.J.R. and Corso, C.R. (2014). Comparative Study of Toxicity of Azo Dye Procion Red MX-58 Following Biosorption and biodegradation Treatments with the Fungi aspergillus niger and Aspergillus terreus. Chemosphere 112 : 317-322

Berbsten-Torralba, L.R., Nishikawa, M.M., Baptista, D.F., Magalhaes, D.P. and da Silva, M. (2009). Decolourization of Different Textile dyes by Penicillium simplicissimum and Toxicity evaluation after fungal Treatment. Brazilian Journal of Microbiology. 40: 808-817.

Bridge, P. and Spooner, P. (2001). Soil Fungi: Diversity and Detection. Plant and Soil. 232: 147-154

Das, S. and Das, B. (2017). Decolourization of Different Food Dyes by 12 Types of Fungi and Toxicity Evaluation After Fungal Treatment. European Journal of Biomedical and Pharmaceutical sciences. 4(8): 1059-1079.

Frac, M., Hannula, S.C., Belka, M. and Jedryczka, M. (2018). Fungal Biodiversity and their Roles in Soil Health. Frontiers in Microbiology. 9: 707

Hadibarata, T., Adnan, L.A., Yusoff, A.R.M., Yuniarto, A.R. and Zubir, M.M.F.A. (2013).Microbial decolourization of an azo dye reactive black 5 using white - rot fungus Pleurotus eryngii F032. Water, Air and Soil Pollution. 224: 1593-1595

Hartikainen, E.S., O. Miettinen, Hatakka, A. and kahkonen, M. A. (2016). Decolourization of six synthetic dyes by fungi. American Journal of Environmental Sciences. 12(2): 77-85.

Hassan, M.A. and El Nemr, A. (2017). Health and Environmental impact of Dyes: Mini Review. American Journal of Environmental Science and Engineering. 1(3): 64-67.

Omar, S.A. (2016). Decolourization of different Textile Dyes by Isolated Aspergillus niger. Journal of Environmental Science and Technology. 9(1): 149-156.

Joshi, P.A., Jaybhaye S. and Mhatre K. (2015). Biodegradation of Dyes Using Consortium of Bacterial Strains Isolated From Textile Effluent. European Journal of Experimental biology. 5(7): 36-40.


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