ANTIBIOTICS SUSCEPTIBILITY PATTERNS OF BACTERIAL ISOLATES FROM UNPASTEURIZED MILK SOLD AT KADUNA STATE UNIVERSITY

Ayokunnumi Funke Obajuluwa, F. A. Muhammad, Z. M. Barau

Abstract


A total number of twenty four (24) random samples of unpasteurized milk were collected from three different locations in Kaduna State University, main campus to assess their microbiological quality and determine the antibiotics susceptibility patterns of the bacteria isolates. Total viable bacteria and coliform counts were carried out using pour plate method. Antibiotics susceptibility test was carried out using Kirby Bauer modified disc diffusion method, while multiple antibiotic resistance (MAR) index of isolates were also determined using recommended procedure. Results obtained revealed that the mean values of total viable bacterial counts ranged from 1.1 x 105 to 2.1 x 105 cfu/ml. Total coliform counts was from 4.6x104 to 7.8x105 cfu/ml. Six different bacteria were isolated from the samples, they were: Bacillus spp (18.8%), E. coli (18.8%), Lactobacillus spp (6.25%), Proteus vulgaris (18.8%), Staphylococcus. aureus (25.0%), and Streptococcus spp (12.5%). Antibiotic susceptibility test of the isolated organisms showed that Gram negative isolates were more resistant to antibiotic action than Gram positive isolates, 18.8% of Gram positive isolates and 75% of Gram negative isolates had MAR index greater than 0.2. The findings of this study showed that the unpasteurized milk samples were contaminated with bacteria including faecal contaminants and that these isolates may have originated from a high risk source of contamination where antibiotics are often used.


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Adam J. Lange, Tracy Ayers, Julian Grass, Michael Lynch, Frederick J. Angulo, Barbara e. Mahon (2012). Non-pasteurized dairy products, disease outbreaks and United State Laws- United States, 1993-2006. Emerging Infectious Diseases. www.cdc.gov/eid. Vol 18, No 3; 385-391.

Akhigbemidu W, Musa A, Kuforji O (2015). Assessment of the microbial qualities of noodles and the accompanying seasonings. Nig. Food J. 33: 48 – 53.

Barnabas BB, Ayodele OP, Gana J, Jiya AG, Igheghe F. (2014). Microbiological assessments of raw milk “Nono” and locally fermented cheese sold at different markets in Bida LGA, Niger State, Nigeria. IOSR Journal of Pharmacy and Biological Sciences. 9(5); 19-22.

Cheesbrough M. (2000). District Laboratory practice in tropical countries part 2 Cambridge low price edition pp 62-70.

Clinical and Laboratory Standards Institute (2012), CLSI- M100S22. Performance Standard for Antimicrobial susceptibility testing: Twenty second International Supplement.

FAO (Food and Agriculture Organization) (2006). Considerations of Food Safety and Consumer Protection http://www.fao.org/docrep/006/y8705e/y8705e09.html] Site visited on 25/8/2017.

Frederick A, Courage K, Setsoafia S, Gabriel AT (2016). Antibiotic Susceptibility of Escherichia coli Isolated from Milk and Hands of Milkers in Nyankpala Community of Ghana. Current Research in Dairy Sciences, 8: 6-11.

Hague S (1997). Food poisoning caused by aerobic spore forming bacilli. J. App!. Bacterio!' 18;591-595.

Karimuribo ED, Kusiluka LJ, Mdegela RH, Kapaga AM, Sindato C, Kambarage DM (2005). Studies on mastitis, milk quality and health risks associated with consumption of milk from pastoral herds in Dodoma and Morogoro regions, Tanzania. Journal of Veterinary Science 6(3): 213 – 221.

Krumperman PH (1983). Appl. Environ. Microbiol. 46: 165-170

Livia A (2006). The effect of fermentation on L (+) and D (-) lactic acid in milk fat. Journal of Diary Science. 65: 521 - 529.

Monica O, Anhi H, Raya M, Tina L, Reijo P, Olu M, Penti H, Pirkko K (2000). A between species comparison of Antimicrobial resistance in Enterobacteria in Faecal flora. Antimicrob. Agents Chemother. 44(16): 1479-1484

Mora A, Blanco JE, Blanc M, Alonso MA, Dhabi GM, Echeita A, Gonzalez EA, Bernardes ML, Blanco J (2005). Antimicrobial resistance of Shiga toxin (verotoxin) producing Escherichia coli 0157: 7 and non-0157 strains isolated from humans, cattle, sheep and food in Spain. Res. Microbiol. 3: 1-14.

Noskin, G.A, Rubin, R.J, Schentag J.J, Kluytmans J, Hedblom EC, Smulders M, Lapetina E, Gemmen E (2005).The Burden of Staphylococcus aureus Infections on Hospitals in the United States: An Analysis of the 2000 and 2001 Nationwide Inpatient Sample Database. Arch Intern Med. 165:1756-1761.

Nwankwo Immaculate, Amaechi Ndubuezeand Adiele, Winnie A. (2015). Microbial evaluation of raw milk from dairy farms in Udi L.G.A.Enugu State, Nigeria. IOSR Journal of Agriculture and Veterinary Science 8(3); 60-65

O’Connor CB, Tripachi BR (2000). Milk processing techniques fermented milk in rural diary processing training series: module 3 international livestock center for Africa publications.

Odu NN, Imaku LN (2013). Assessment of the Microbiological Quality of Street-vended Ready-To-Eat Bole (roasted plantain) Fish (Trachurus trachurus) in Port Harcourt Metropolis, Nigeria. Researcher, 5(3): 9-18.

Oladipo IC, Tona GO, Akinlabi EE, Bosede OE (2016). Bacteriological quality of raw cow’s milk from different dairy farms in Ogbomoso, Nigeria. International Journal of Advanced Research in Biological Sciences 3(8).

Osundiya O. O, Oladele R. O., Oduyebo O. O (2013). Multiple antibiotic resistance (MAR) indices of Pseudomonas and Klebsiella species isolates in Lagos University Teaching Hospital. Afr. J. Cln. Exper. Microbiol. 14(3): 164-168

Pehrsson P.R, Haytowitz D.B, Holden JM, Perry CR, Beckler DG (2000). USDA’s National Food and Nutrient Analysis program: food sampling. Journal of Food Composition Analysis 13(4): 379-389.

Saba R, Muhammad F., Shahida H. (2011). Antibiotics susceptibility pattern and multiple antibiotic resistance (MAR) calculation of extended spectrum β-lctamase (ESBL) producing Escherichia coli and Klebsiella spesies in Pakistan. Afr. J. Biotechnol; 10(33): 6325-6331.

Salton, Milton RJ. (1996). “Structure”. Medical Microbiology 4th Edition U.S. National Library of Medicine.

Sanders, E.R. (2012). Aseptic Laboratory Techniques: Plating Methods. Journal of Visual Experiments, 63: e3064. doi: 10.3791/3064

Swai, E.S. and Schoonman, L. (2011). Microbial quality and associated health risks of raw milk marketed in the Tanga region of Tanzania, Asian Pacific Journal of Tropical Biomedicine 1(3): 217 – 222.

Unnevehr, L. and Hirschhorn, N. (2000). Food safety issues in the developing world. World Bank Technical Paper No. 469. The World Bank, Washington DC. 72


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