IMPROVEMENT OF NODULATION AND NITROGEN FIXATION OF GROUNDNUT (Arachishypogaea L.) USING PASTURE AND GRAINLEGUME RHIZOBIA ISOLATES INDIFFERENT SOILMANAGEMENT.

S M YAHAYA, I A ALIYU, S K BELLO

Abstract


A greenhouse experiment was conducted to examine the effect of pasture (Centrosema pubescence, Centrosemapascorum, Mycrotlomauniflorum, and Mucuna pureness) and legume (Vigna unguiculata-2, Vigna subterranean-2, and Arachishypogaea) rhizobia isolates on nodulation and nitrogen fixation of groundnut (Samnut 24) under two different soil types i.e. cultivated soil (CS) and fallowed soil (FS). Fresh nodules were obtained, crushed and rhizobia isolates extracted which were later inoculated via liquid inoculation to groundnut. All agronomic practices were followed and harvesting was done at eight week after planting. Results revealed that response of groundnut to both pasture and legume rhizobia inoculation was significantly higher (p< 0.0001) in CS than in FS in all the parameters measured with the exception of root dry weight, this may be due to the fact that the experiment was conducted in pot which limit root growth. Significant differences were observed between the isolates in terms of number of branches, number of pegs, number of pods, root length, nodule number, and phosphorus uptake,with one isolates or the other performing better than the un-inoculated control. Significant soil versus isolate interactions was also observed in terms of plant height, number of branches, root length, root dry weight, and phosphorus uptake. Correlation analysis showed that nodule number significantly correlated with shoot dry weight, number of branches, number of pods, number of pegs, nodule dry weight, dry matter yield, and ultimately nitrogen fixation  (r = 0.66, 0.76, 0.64, 0.74, 0.92, 0.66, and 0.54 respectively)

Full Text:

PDF

References


Ahmad, E., Khan, M.S. and Zaidi, A. (2013).ACC deaminase producing Pseudomonas putida strain PSE3 and Rhizobium leguminosarum strain RP2 in synergism improves growth, nodulation and yield of pea grown in alluvial soils, Symbiosis, 61(2), 93-104.

Anderson, J.M. and Ingram, J. (1994).Tropical soil biology and fertility: a handbook of methods, Soil Science, 157(4), 265.

Bashan, Y., de-Bashan, L.E., Prabhu, S. and Hernandez, J.P. (2014).Advances in plant growth-promoting bacterial inoculant technology: formulations and practical perspectives (1998–2013), Plant and Soil, 378(1-2), 1-33.

Catroux, G., Hartmann, A. and Revellin, C. (2001) Trends in rhizobial inoculant production and use, Plant and soil, 230(1), 21-30.

Drevon, J.J., Abadie, J., Alkama, N., Andriamananjara, A., Amenc, L., Bargaz, A., Carlssonn, G., Jaillard, B., Lazali, M. and Ghoulam, C. (2015).Phosphorus use efficiency for N2 fixation in the rhizobial symbiosis with legumes, Biological nitrogen fixation. Wiley, Hoboken, 455-464.

Enwezor, W., Ohiri, A., Opuwaribo, E. and Udo, E. (1990).Literature review on soil fertility investigations in Nigeria, Federal Ministry of Agriculture and Natural Resources, Lagos. P, 281.

Fox, J. and Andersen, R. (2005) Using the R statistical computing environment to teach social statistics courses, Department of Sociology, McMaster University, 2-4.

Gee, G.W. (2005) TEXTURE, In: Hillel, D. (ed.) Encyclopedia of Soils in the Environment. Oxford: Elsevier, 149-155.

Gentili, F. and Huss‐Danell, K. (2003).Local and systemic effects of phosphorus and nitrogen on nodulation and nodule function in Alnus incana, Journal of Experimental Botany, 54(393), 2757-2767.

Giller, K.E. (2001).Nitrogen fixation in tropical cropping systems. Cabi.

Hatfield, J., Egli, D., Leggett, J. and Peaslee, D. (1974).Effect of Applied Nitrogen on the Nodulation and Early Growth of Soybeans (Glycine Max (L.) MERR.) 1, Agronomy Journal, 66(1), 112-114.

Hungria, M, and Campo, R. (2005). Fixação biológica do nitrogênio em sistemas agrícolas. In Congresso brasileiro de ciência do solo.(30).

IITA (1982).Automated and semi-automated methods for soil and plant analysis. International Institute of Tropical Agriculture.

Imran, A., Mirza, M.S., Shah, T.M., Malik, K.A. and Hafeez, F.Y. (2015).Differential response of kabuli and desi chickpea genotypes toward inoculation with PGPR in different soils, Frontiers in Microbiology, 6, p. 859.

Jones, M. and Wild, A. (1975).Soils of the West African savanna. Tech. Comm. No. 55. Commonwealth Bureau of Soils. Harpenden, UK, Agbenin J O. Phosphorus sorption by three cultivated savanna Alfisols as influenced by pH. Fert. Res, 44, 107-112.

Jordan, D. (1982).Transfer of Rhizobium japonicum Buchanan 1980 to Bradyrhizobium gen. nov., a genus of slow-growing, root nodule bacteria from leguminous plants', International Journal of Systematic and Evolutionary Microbiology, 32(1), 136-139.

Khaitov, B., Kurbonov, A., Abdiev, A. and Adilov, M. (2016).Effect of chickpea in association with Rhizobium to crop productivity and soil fertility', Eurasian Journal of Soil Science (EJSS), 5(2), 105-112.

Kuang, R.B., Liao, H., Yan, X.L. and Dong, Y.S. (2005).Phosphorus and nitrogen interactions in field‐grown soybean as related to genetic attributes of root morphological and nodular traits, Journal of Integrative Plant Biology, 47(5), 549-559.

Mapfumo, P., Mtambanengwe, F., Giller, K. and Mpepereki, S. (2005).Tapping indigenous herbaceous legumes for soil fertility management by resource-poor farmers in Zimbabwe, Agriculture, Ecosystems &Environment, 109(3-4), 221-233.

Marschner, H. (1995).Saline soils, Mineral Nutrition of Higher Plants, 657-680.

Marx, E., Hart, J. and Stevens, R. (1999).Soil Test Interpretation Guide Oregan state University Press, Oregan, USA.

Mathers, H., Lowe, S., Scagel, C., Struve, D. and Case, L. (2007).Abiotic factors influencing root growth of woody nursery plants in containers, HortTechnology, 17(2), 151-162.

Novozamsky, I., Houba, V., Van Eck, R. and Van Vark, W. (1983).A novel digestion technique for multi‐element plant analysis, Communications in Soil Science and Plant Analysis, 14(3), 239-248.

Okalebo, J.R., Gathua, K.W. and Woomer, P.L. (2002).Laboratory methods of soil and plant analysis: a working manual second edition, TSBFCIAT and SACRED Africa. Nairobi, Kenya.76 - 78.

Oliveira, R.S., Carvalho, P., Marques, G., Ferreira, L., Pereira, S., Nunes, M., Rocha, I., Ma, Y., Carvalho, M.F., Vosátka, M. and Freitas, H. (2017).Improved grain yield of cowpea (Vigna unguiculata) under water deficit after inoculation with Bradyrhizobium elkanii and Rhizophagus irregularis, Crop and Pasture Science, 68(11), 1052-1059.

Pang, J., Ryan, M.H., Lambers, H. and Siddique, K.H. (2018).Phosphorus acquisition and utilisation in crop legumes under global change, Current Opinion in Plant Biology, 45(B) 248 -254

Pearson, E.J., Juggins, S. and Tyler, J. (2014).Ultrahigh resolution total organic carbon analysis using Fourier Transform Near Infrarred Reflectance Spectroscopy (FT-NIRS), Geochemistry, Geophysics, Geosystems, 15(1), 292-301.

R_Core_Team (2014).R: A language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing; 2014.

Searle, P.L. (1984).The Berthelot or indophenol reaction and its use in the analytical chemistry of nitrogen. A review, Analyst, 109(5), 549-568.

Sims, J.T., Kovar, J. and Pierzynski, G. (2009).Soil test phosphorus: Principles and methods', Methods of phosphorus analysis for soils, sediments, residuals and waters, 2nd edn. Southern Cooperative Series bulletin, 408, 9-19.

Somado, E.A., Becker, M., Kuehne, R.F., Sahrawat, K.L. and Vlek, P.L. (2003).Combined effects of legumes with rock phosphorus on rice in West Africa, Agronomy Journal, 95(5), 1172-1178.

Somasegaran, P. and Hoben, H.J. (1985) Methods in legume-Rhizobium technology. University of Hawaii NifTAL Project and MIRCEN, Department of Agronomy and Soil Science, Hawaii Institute of Tropical Agriculture and Human Resources, College of Tropical Agriculture and Human Resources.

Sprent, J.I., Ardley, J. and James, E.K. (2017).Biogeography of nodulated legumes and their nitrogen-fixing symbionts, New Phytologist, 215(1), 40-56.

Tang, C., Robson, A. and Dilworth, M. (1992).The role of iron in the (Brady) Rhizobium legume symbiosis, Journal of Plant Nutrition, 15(10), 2235-2252.

Vargas, L.K., Volpiano, C.G., Lisboa, B.B., Giongo, A., Beneduzi, A. and Passaglia, L.M.P. (2017).Potential of Rhizobia as Plant Growth-Promoting Rhizobacteria, Iin Zaidi, A., Khan, M.S. and Musarrat, J. (eds.)Microbes for Legume Improvement. Cham: Springer International Publishing, 153-174.

Velázquez, E., Carro, L., Flores-Félix, J.D., Martínez-Hidalgo, P., Menéndez, E., Ramírez-Bahena, M.-H., Mulas, R., González-Andrés, F., Martínez-Molina, E. and Peix, A. (2017)'The Legume Nodule Microbiome: A Source of Plant Growth-Promoting Bacteria', in Kumar, V., Kumar, M., Sharma, S. and Prasad, R. (eds.) Probiotics and Plant Health. Singapore: Springer Singapore, 41-70.

Voravit, R. and Somabhi, M. (1989)'Study on nitrogen fixation by rhizobium in groundnut', Thai J. Soils and Fert, 10, 368-379.

Yusif, S., Muhammad, I., Hayatu, N., Haliru, M., Mohammed, M., Hussain, A. and Fardami, A. (2016)'Effects of biochar and rhizobium inoculation on selected soil chemical properties, shoot nitrogen and phosphorus of groundnut plants (arachis hypogaea l.) in sokoto state, nigeria', Journal of Advances in Biology & Biotechnology, 8(2), pp. 1-6.

Zaman-Allah, M., Sifi, B., L'taief, B., El Aouni, M. and Drevon, J. (2007)'Rhizobial inoculation and P fertilization response in common bean (Phaseolus vulgaris) under glasshouse and field conditions', Experimental Agriculture, 43(1), 67-77.

Ziaf, K., Latif, U., Amjad, M., Shabir, M.Z., Asghar, W., Ahmed, S., Ahmad, I., Jahangir, M.M. and Anwar, W. (2016)'Combined use of microbial and synthetic amendments can improve radish (Raphanus sativus) yield', J Environ Agric Sci, 6, . 10-15.


Refbacks

  • There are currently no refbacks.