A greenhouse experiment was conducted to examine the effect of pasture (Centrosema pubescence, Centrosemapascorum, Mycrotlomauniflorum, and Mucuna pureness) and legume (Vigna unguiculata-2, Vigna subterranean-2, and Arachishypogaea) rhizobia isolates on nodulation and nitrogen fixation of groundnut (Samnut 24) under two different soil types i.e. cultivated soil (CS) and fallowed soil (FS). Fresh nodules were obtained, crushed and rhizobia isolates extracted which were later inoculated via liquid inoculation to groundnut. All agronomic practices were followed and harvesting was done at eight week after planting. Results revealed that response of groundnut to both pasture and legume rhizobia inoculation was significantly higher (p< 0.0001) in CS than in FS in all the parameters measured with the exception of root dry weight, this may be due to the fact that the experiment was conducted in pot which limit root growth. Significant differences were observed between the isolates in terms of number of branches, number of pegs, number of pods, root length, nodule number, and phosphorus uptake,with one isolates or the other performing better than the un-inoculated control. Significant soil versus isolate interactions was also observed in terms of plant height, number of branches, root length, root dry weight, and phosphorus uptake. Correlation analysis showed that nodule number significantly correlated with shoot dry weight, number of branches, number of pods, number of pegs, nodule dry weight, dry matter yield, and ultimately nitrogen fixation  (r = 0.66, 0.76, 0.64, 0.74, 0.92, 0.66, and 0.54 respectively)

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